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The ceiling and walls are simply a PVC-coated polyester membrane that is held aloft by air from giant fans.
For in vitro cellular permeability studies, Caco2 cells or HT29 cells (2 × 104 cells/cm2) were seeded in 24-well Transwell® plates (Cornings; USA), on polyester membrane filters (pore size 0.4 µm, surface area 1.12 cm2)60.
First, the basal chamber of the transwell inserts was filled up with 1.5 ml of cell culture medium on day 0. Next, 1 * 105 cells cm−2 in 0.5 ml culture medium were seeded onto the 3 μm porous polyester membrane of the apical chamber.
This work presents an investigation of the chemical aging of a commercial nonwoven polyester membrane under different temperatures and pH environments in relation to its morphology, mechanical properties and molar mass.
We have found that a simple commercial polyester membrane when coated with gold and polarised to −4 V (versus Ag) in aprotic organic solvents displays an electrochromic colour change from a colourless to an intense red state.
Drilling mud pits were stabilized by mixing with clean soil, covering with a polyester membrane, topped with soil and re-seeded.
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They consisted of two polyester membranes with a water-filled gap between.
Hayes and coworkers improved on this method by growing human cells on Snapwell™ brand permeable polyester membranes.
Cell migration assays were performed in Corning Transwell®-96 permeable support chambers with porous polyester membranes with a pore size of 8.0 μm (Corning Incorporated Life Sciences, Munich, Germany).
Polyester membranes with a pore size of 1.3 μm were used and incubation was performed at 37°C in a 5% CO2 atmosphere for 48 h.
Briefly, Transwell® inserts (clear polyester membranes with 0.4 μm pore size) were seeded with human primary astrocytes (HA, passage no. 3-4) on the abluminal side of the microporous membrane and cultured in DMEM/F12 media with 10% FBS.
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