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The safety and utility of polscope imaging has been demonstrated in mammalian oocytes, including those from women undergoing ICSI.
Spindle imaging with the polscope provides structural information closely related to the more invasive immunostaining method, and also enables study of the dynamic architecture of spindles.
A time-lapse video of a mouse oocyte imaged by polscope may be purchased for viewing on the internet at www.rbmonline.com/Article/824 (free to web subscribers).
Profound effects of cooling on meiotic spindles have also been shown, and polscope imaging has been used to optimize thermodynamic stability of oocytes during ICSI.
Polarized light microscopy has been gainfully applied to embryology for decades, but recently a digital, orientation-independent polarized light microscope, the polscope, has demonstrated the exquisite sensitivity needed to image the low levels of birefringence exhibited by mammalian spindles.
We have imaged protozoa and other small metazoans using differential interference contrast (DIC) microscopy, orientation-independent (OI) DIC, and real-time birefringence imaging using a video-enhanced polychromatic polscope.
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Objective: To examine whether spindle morphologic features imaged with the LC-PolScope (Cambridge Research and Instrumentation, Woburn, MA) in living human oocytes matured in vitro can be used to predict chromosome configuration and select oocytes with normal chromosomes.
Objective: To examine the effects of different thermodynamic control systems on the temperature stability of human eggs during in vitro manipulation, with the integrity of meiotic spindles imaged using the LC-PolScope (Cambridge Research & Instrumentation, Inc., Woburn, MA).
Images were acquired using an Olympus BX51 microscope equipped with Olympus BX series circular polarizer/interference filters, a liquid crystal compensator and CCD camera, and processing software (Abrio LC-PolScope, Cambridge Research & Instrumentation, Woburn, MA, USA).
Semi-thin sections (0.5 μm) were cut on an ultra-microtome, mounted in immersion oil, and observed through a microscope equipped with the LC-PolScope (CRI Cambridge MA) as described previously [ 49].
The CRI PolScope always needs a reference region with no birefringence within the FOV.
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