This hand-pollination procedure was chosen to approximate the behaviour of a bumble bee in a patch of oilseed rape.
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Without the petals removed, it was impossible for insects to do any pollinating.Why did the honey-bush evolve such a complicated procedure for its pollination?
Total RNA was isolated from immature kernels 8, 14, 20, 26 and 32 days after pollination (DAP) using a modified procedure of Prescott and Martin [ 64].
Tong211 and a dicot plant, evening primrose (Oenothera biennis L ., and followed by self-pollination with pollens of different individuals of the same rice cultivar, namely, using a procedure we termed "repeated pollination" [ 27, 40].
We used a standard procedure that we developed for pollination drops, complete details of which were published in a paper by Wagner et al. (2007).
Controlled pollination was achieved by the use of standard breeding procedures.
An efficient manual crossing procedure has been developped based on a microscopic study of pollination and fertilization kinetics in relation to the intact flower morphology of M. truncatula (E.-P. Journet, unpublished).
