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This may be explained by incomplete pollination, post-pollination barriers or embryo development problems.
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Both the male and female organs of the flower are especially sensitive to temperature fluctuations both during their development before pollination and during the post-pollination stage.
However, our results with 1-day old styles differ slightly in that S-RNase and HT-A transcript levels respond differentially to compatible pollination, suggesting that at shorter times post-pollination, effects of senescence and pollination may be distinguished.
Interestingly, ethylene production following pollination occurs in two bouts, one several hours post-pollination that is independent of the type of pollination and a second about one day post-pollination that occurs only in compatible pollinations [46].
At 27 hours post-pollination, some fluorescence can be observed at the bottom of the styles, but the pollen tubes do not emerge until 28 hours post-pollination (Figure 3A).
Interestingly, pollination-induced changes in the flower include changes in transmitting tract cell morphology [44] that are visible within a day post-pollination, while responses to the senescence hormone ethylene involves changes in the entire flower.
The fertilization success of crop pollen could also be limited by post-pollination mechanisms.
One-way ANOVA was performed to compare the angles per flower pre- and post-pollination.
8 months post-pollination pods were freeze-dried and crushed into a fine powder.
Maize can be crossed ∼60 days after planting and requires another 30 45 days post-pollination for seed maturation.
It is generally assumed that sexually deceptive orchids are strongly isolated via pre-pollination barriers, and more weakly through post-zygotic (i.e., post-pollination) mechanisms, which contrasts to the situation observed in food deceptive orchids that have weak pre-pollination [ 62] but strong post-zygotic isolation barriers [ 63].
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