Apples are self-incompatible; they must cross-pollinate to develop fruit.
One BC3F1 plant was selected and self-pollinated to develop the NIL with IRRI 146 background (Fujita et al. 2013).
T0 plants were self pollinated to obtain T1 seeds.
For oilseed hand pollinated plants, the first 30 flowers to develop on the primary stem were supplementary pollinated, with pollen from 5 donor plants.
The moths actively pollinate to ensure a food source for their developing offspring.
Flowers were pollinated and tagged at anthesis and one fruit was allowed to develop per plant.
The fruit takes ∼11 14 months to develop after the receptive female flower is successfully pollinated and before the growing point of the embryo penetrates the germ pore (Marar and Varma 1958).
Female flowers were hand pollinated with male flowers from the same plants, bagged and the fruits left to develop to maturity.
Low-P tolerant cell lines were obtained and the regenerated plants (R0) were self-pollinated to harvest seeds.
Putative mutants were isolated and allowed to self-pollinate to obtain seed.
This indicates that the potential fecundity offered by a large number of small flowers was offset by the reduced likelihood that each of these flowers would be pollinated and develop into a mature fruit.
