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Results from this study show that constructed wetlands have the potential to be developed into an environmentally acceptable river water quality improvement and wastewater polishment alternative for practical application.
The cDNA for dominant negative (DN) FADD (clone AU1-NFD4, kindly provided by Dr. C. Vincenz, University of Michigan, Ann Arbor, USA) was released from pcDNA3 (Invitrogen, Karlsruhe, Germany) with HindIII and XhoI followed by polishment of both ends with Klenow fragment and subcloning into the PmeI site of the retroviral vector TJBA5bMolink-neo (Howard et al, 2000).
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