Sentence examples for pole distance from inspiring English sources

For pole to pole distance and spindle width measurements, at least 3 independent experiments were performed with at least 20 cells per condition measured in each experiment.

While siRNA-mediated CRMP4 knockdown had no effect on spindle width (Fig. 10C), we did observe a significant decrease in the pole to pole distance compared to control treated cells (Fig. 10A and 10B).

However, in many cells the pole to pole distance was shorter than in wild type cells indicating nuclear microtubule defects that we further investigated by thin section electron microscopy (EM).

We performed a morphometric analysis of the mitotic spindle by measuring spindle length (pole to pole distance) and spindle width 9 hours following release from a double thymidine block (Fig. 10A 10C), as described previously with GSK3 inhibitors [33].

For pole to pole distance measurements, tubulin fluorescence intensities were measured from one end of the cell to the other end along the spindle axis using ImageJ, and when plotted as a function of spindle position, the tubulin intensity gave two peaks corresponding to the spindle poles (Fig. S3) [33].

During mitosis, Kinesin-5 motors function near the spindle midzone to maintain pole to pole distance.

Going back more than 30 million years, however, successively greater "virtual pole" distances are revealed, indicating that substantial deviations occurred.

The strains contained SPC42 -3XGFP and PDS1 -13XMyc to facilitate monitoring spindle pole distances and entrance into anaphase, respectively.

To calculate spindle pole distances, we wrote a Matlab script that identifies Spc110-mCherry foci and calculates a distance to the nearest neighbor for each instance.

We found that an scc4 m3 -bearing strain exhibited increased inter-spindle pole distances and that the scc4 mutationion did not exacerbate the spindle extension phenotype of a chl4Δ strain.

We found no evidence to suggest that disruptions to these traits influenced host parents' likelihood to eject or the latency to ejection, which suggests that in Polačiková et al. (2013) ejecter females that maintained consistent arrangement of blunt pole distances and adjacent angles were not using these as independent cues.