The results suggest the possibility of designing experiments using combinations of tailored polarization sequences to separate and monitor individual relaxation pathways.
For all samples, a cross-polarization sequence, optimized to obtain semiquantitative data, was used with a 1.0 ms contact time and a pulse delay of 4 s.
The cross polarization pulse sequence was applied with a composite shaped "ramp" pulse on the 1H channel in order to account for the inhomogeneity of Hartmann-Hann condition at high rotor spin frequency.
As seen in a polarization microscopy sequence (Fig. 2B; Video S2, n = 15), the microtubules of the newly folded central spindle (0 min, equivalent to schematic 2A, d) began to reorganize, lengthening from the joint poles toward the cortex (0 11 min, arrows).
The reorganization of the collapsed spindle was captured in a polarization microscopy sequence (Fig. 1B; Video S1; n = 12), which showed the mitochondria being pushed outward by two bundles of laterally protruding microtubules (Fig. 1B; 5 min; microtubules appeared as black fibers).
A direct polarization pulse sequence was used to record the spectra, with a long recycle (300 s).
By combining a recently developed cross-polarization pulse sequence designed for quantitative analysis of even low hydrogen containing materials with long range dipolar dephasing, we examine the structure of a thermoseries of chars produced from cellulose.
The inversion-recovery cross-polarization (IRCP) sequence used for measuring cross-relaxation time (TCH) is modified to obtain signals that show exponential or spin-lock (SL) decay to zero.
The spectra were measured under proton decoupling during acquisition and with DEPT polarization transfer pulse sequence.
Mishkovsky et al. have demonstrated that by using uniformly C-enriched acetate and a polarization transfer pulse sequence, the metabolism of acetate to the level of α-ketoglutarate can be detected in the rat brain.
Data acquisition was performed using a cross-polarization (CP) pulse sequence, i.e. a 2.95 μs proton 90 degree pulse and an 800-μs ramped (100 to 50%) falling contact pulse, with a 2.5 s delay between repetitions.
