Sentence examples for polarization assays from inspiring English sources

For macrophage polarization assays, BMDMs were cultured with M1-polarizing conditions (primed overnight with interferon [IFN]-γ 100 ng/ml and stimulated for 3 h with LPS [10 ng/ml]) and M2-polarizing conditions (stimulated for 24 h with IL-4 [40 ng/mL]) with or without Nec-1 (30 μM) and/or Z-Ile-Glu O-ME -Thr-Asp O-Me -FMK (Z-Ile-Glu O-ME -Thr-Asp O-Me -FMK).

Four of these scFvs bound to free monensin as determined using competitive fluorescence polarization assays (C-FPs).

To validate whether AEBP2 ZF1–3 binds to double stranded DNA (dsDNA), we conducted fluorescence polarization assays with the 44-bp GC-rich, AT-rich and previously reported T1 dsDNA (Kim et al., 2009).

We also employ fluorescence polarization assays to substantiate the computational findings; it is found that the rationally designed peptides exhibit moderate or high affinity to TnC with dissociation constants KD at micromolar level.

Here, by using a combination of molecular modeling, NMR-based structural analysis, fluorescence polarization assays, and cell-based assays, we have designed and characterized a novel proapoptotic compound targeting these proteins.

Fluorescence polarization assays showed that peptide 1 had a higher binding affinity (Kd = 0.6 μM) to the Src SH2 domain when compared with Ac-pYEEI (Kd = 1.7 μM), an optimal Src SH2 domain ligand, and peptides 2 4 (Kd = 2.9 52.7 μM).

ER binding affinities were evaluated by a fluorescent polarization assay with the corresponding inactive In III) complex or iodinated peptide.

These compounds were tested for binding to the XIAP-BIR3 and ML-IAP BIR using a fluorescence polarization assay.

Screening of ligands in a VDR fluorescence polarization assay and a RXR/VDR conformation sensing assay resulted in the identification of multiple fragment hits (lean >0.30).

Herein, we present chemical evidence that bestatin ester analogs directly interact with the cIAP1-BIR3 domain by means of fluorescence polarization assay and photoaffinity labeling assay using fluorescent probes.

To compare the usefulness of a lamellar body count, a fluorescence polarization assay, and the foam stability index for predicting neonatal lung maturity in high-risk pregnancies.