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Comparison of the direct excitation spectra (Fig. 2A/B) with H-P cross-polarisation (data not shown) showed no significant differences in the relative intensities of the resonances, in agreement with crystallographic studies, indicating that the phosphate does not undergo a significant reduction in mobility upon binding to the non-annular binding site.
In addition, conjugation with 4-fluorobenzaldehyde (AH113804) was found to have no relevant effect on the binding of AH111972 to human c-Met (K i of unlabelled AH113804 was not significantly different to that of AH111972 tested against GE-137 in a fluorescence polarisation competition assay; data not shown).
Prolonged exposure to a signal or signal combination tends to increase the response in terms of expression but has a negative effect on polarisation of the expression (Fig. 6 and data not shown).
p.i (data not shown).
(Data not shown).
(detailed data not shown).
SLS showed similar results (data not shown).
However, dystrophin was not (data not shown).
rapa genome synteny (data not shown).
were unsuccessful so far (data not shown).
Second, seedlings were shorter (data not shown).
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