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After indicated time points, whole cell lysates were prepared with radioimmunoprecipitation assay (RIPA) lysis buffer (Upstate, Charlottesville, VA, USA) containing Protease Inhibitor Cocktail (Roche, Mannheim, Germany).
For determination of virus lung titers and ISG mRNA profiles, animals were sacrificed at indicated time points, whole lungs were collected, homogenized in 500 μl PBS, and debris was spun down at 15,000 rpm for 10 min.
At various time points, whole blood was isolated from the tail vein of recipients as described (see above under 'Platelet clearance analysis') and the disappearance of Alexa488+ platelets analysed by flow cytometry.
At various time points, whole blood was isolated from the tail vein and mixed with Resuspension Buffer Mix, which comprised 25% (v/v) Aster Jandl Anticoagulant (85 mM sodium citrate dihydrate, 69 mM citric acid anhydrans, 10 mM glucose, pH 4.6) and 75% (v/v) Resuspension buffer (10 mM HEPES, 140 mM NaCl, 3 mM KCl, 0.5 mM MgCl2 hexahydrate, 0.5 mM NaHCO3, 10 mM glucose, pH 7.4).
Similar(56)
With all that finger pointing the whole point is missed.
Generality is the whole point.
That is the whole point.
Its hardness was the whole point.
The whole point is not knowing.
The whole point of LudoBites is surprise.
That was the whole point of it".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com