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The cell lysates collected at different time points were subjected to Western blotting assay as above.
Only pre-defined end points were subjected to statistical testing using a 5% (two-sided) significance level.
However, this should not affect the validity of the data analysis, as all the samples collected from different time points were subjected to the same DNA extraction method.
Proteins collected at the indicated time points were subjected to immunoblotting with antibodies to HA. Image shown represent example of immunoblotting and graphs, the quantification of three experiments.
DE genes (254) common to all time points were subjected to hierarchical clustering and Short Time series Expression Miner (STEM) analysis.
Proteins obtained from the two infection time points were subjected to the proteomic analysis and three technical replicates were performed for each of two biological replicates.
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The massive material points are subjected to the elastic force of solid deformation but do not interact with the fluid directly, while the massless material points interact with the fluid by moving with the local fluid velocity.
EndoSequence BC points are subjected to a patented process of impregnating and coating each cone with bioceramic nanoparticles.
The sampled cell culture (1.5 ml) at different time points was subjected to centrifugation at 4,500 × g for 10 min at 4°C.
A pooled list of RefSeq accession numbers for transcripts exhibiting differential expression between Wt and Nr2e1 frc / frc genotypes, at least at one of the three different time points, was subjected to an oPOSSUM TFBS analysis.
The filtered significantly down regulated genes (Log2 (fold change) < −2 and P value < 0.05) of each time point were subjected to GO enrichment analysis separately in biological process, molecular biology, and cellular function.
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