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Data recorded on the same patients but at different time points were compared by the Wilcoxon signed rank test.

Quantitative variables collected at baseline and 60 min after the procedure or at baseline and the 5- and 60-min time points were compared by Student's t test, analysis of variance for repeated measurements, or non-parametric tests, as appropriate.

The precursor enrichments at the single time points were compared by a two-way ANOVA with repeated measures.

Categorical end points were compared by using the chi-squared test.

The means between groups at different time points were compared by two-way ANOVA.

Values at single time points were compared by one-way ANOVA followed by Duncan's post hoc test.

The treatment mean values at same time point were compared by post hoc least significant difference (LSD) test.

The mean values detected in the two groups at each time point were compared by t test.

The groups at a given time point were compared by 1-way ANOVA followed by Bonferroni post test (for three groups) or unpaired, two-tailed t test (for two groups) using Graph Pad Prism Version 5.00 for Windows.

Concentrations at a single time point were compared by using Wilcoxon rank sum test.

Differences between the levels of serum C1M at each time point were compared by two-way ANOVA.