At each time point, we searched for three different places with leukocyte infiltration in mouse ear pinna.
For each data point, we searched for nearby data points (the 'search area' was a circle with a radius of 100, 200 or 400 km around the data point) and we calculated the proportion of data points that were cases in each search area.
Since our experimental setting included the comparative analysis of global gene expression at two time points (3 or 60 hours), we searched for genes that best differentiate our samples as a function of time.
Briefly, after 15 min on supine decubitus and previous application of topical anesthetic, IOP was measured with the Shiotz tonometer, with 5.5 and 7.5 weights; there is a modification of the technique, where these two measures are compared with the IOP taken with Goldman tonometer; finally, in the Friedenwald's nomogram, we searched the point where both IOP measurements intersect.
Finally, in order to further evaluate a potential tumor suppressor genetic model we searched for point mutations of the NOTCH2 gene in four of our OD cases.
To confirm this point, as done with Lhcb3, we searched in the public databases for sequences having significant similarity with Lhcb6 in algae and non-seed plants.
We searched for a point source in both the community and hospital settings.
Now we reach the point where we search for an appropriate open bounded subset Ω for the application of the continuation theorem (Lemma 2.1).
Now, we reach the point where we search for appropriate open bounded subsets (Omega_{i}), (i=1,2,ldots,8), for the application of the continuation theorem.
With an initial point, we can start to search for a local minimizer.
