Sentence examples for points under puromycin from inspiring English sources

At later time points, under puromycin selection, all of the populations displayed higher fractions of apoptotic cells.

Expression of ARzL, the ribozyme with two long antisense arms, cannot be enriched under puromycin without killing the transfected cells, probably due to excessive depletion of ADHE.

Expression of the ribozyme with two short antisense arms, ARzS, was stabilized under puromycin selection and demonstrated a 33% reduction in ADHE mRNA and 25% decrease in NAD+-dependent ADH activity in the transfectants.

Using selection media under puromycin, Snail shRNA and Slug shRNA transfected A2780 cell clones revealed a transfection efficiency of 99% stable knockdown as determined using RT-PCR analysis using total RNAs from parental A2780 and Snail and Slug shRNA knockdown cells.

One day later cells were passaged under puromycin selection (10 µg/ml).

To prevent premature growth of in situ switchers, NEO-tagged clones were grown under Puromycin selection.

Stable Cyp KD cell lines were obtained under puromycin (1 µg/ml) selection and CypA KD was verified by Western blotting as described previously [9].

Cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum, 1% glutamine and 5×10−5 M β-mercaptoethanol (Sigma) under puromycin (1.5 µg/mL) selection.

Shed virus was harvested 48 hours after transfection, and control or stable SALL4 knockdown H9 clones were obtained under puromycin (1.2 µg/mL) selection after 7 days.

HFFs were infected with retrovirus stock to generate a population of the cells expressing EGFP or EGFP- UL76 fusion protein under puromycin selection.

Cells were maintained under puromycin (Sigma) selection.