(a) Bar graph representing the expression pattern of the fluorescent protein (YFP) and OX40L after transfection of eYFP-C1-OX40L into RPE at five time points (time point 1 = 30 min, time point 2 = 1 h, time point 3 = 4 h, time point 4 = 6 h, and time point 5 = 24 h).
No join point (time point when a significant trend change is detected) resulted from the trend analysis.
The normalized threshold value (ΔCt) of each gene along time course was compared to that of the same gene at the first time point (time point A in Figure 1A).
Likewise, for the HacACA strain three bioreactor cultivations were performed and biomass was harvested from each culture and RNA was isolated from the mid-exponential time point (time point 1; HacACA-1; [glucose] = 5.7 g/L).
However, the number of nodules in gp130Δhepa livers was not significantly decreased at this time point, time point suggesting that the differences observed after acute DEN injection likely have no impact on tumor initiation.
Data were collected at four time-points: Time-point 1 at the time of diagnosis; time-point 2 during the treatment; time-point 3 after the child had completed their treatment and time-point 4 when the child had been off treatment for some years or had died.
Open image in new window Fig. 2 Inspection points, transition points and time points.
(A) The line chart was generated with microarray data of mRNA products of all time points (three GROWTH time points, seven STARVATION time points and ten conjugation time points) in life cycle of T. thermophila.
Figure 2 shows the inspection points, state transition points and time points, where (Delta_{l}) means the lth inspection point, S1 and S2 represent two different situations, respectively.
The three time points were randomly chosen out of four predefined time points: immediately after the presentation (time point 1), after 2.5 h (time point 2), after 24 h (time point 3) or after 2 weeks (time point 4).
