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The embryos were grown at 28°C for the selected time points (stages), 8 hours post-fertilization (hpf) (60 75% epiboly), 14 hpf (8 10 somite), 24 hpf (prim-5), and 72 hpf (protruding mouth).
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Stage I (minimal) 1 5 points; Stage II (mild) 6 15 points; Stage III (moderate) 16 40 points; Stage IV (severe) >40 points.
The summarized resulting point scores are classified into four grades of severity: Stage I (minimal) 1 5 points; Stage II (mild) 6 15 points; Stage III (moderate) 16 40 points; Stage IV (severe) >40 points.
The required pointing accuracy will be achieved by combining coarse (ADCS) and fine (OBT/BSM) pointing stages.
(AP) COACH'S RESIGNATIONS BAFFLES PANTHERS: As bad as Carolina's offense has been this season, things had yet to deteriorate to the finger-pointing stage.
We measured embryos at stages 14 and 15, following the first point (stage 13)17,20 at which the pallium and subpallium can be distinguished by cellular boundaries (for example, the PSB).
Rotherham, who started the season with a 17-point deduction, got themselves to the plus-one point stage as they came from behind for a 3-1 win over struggling Grimsby.
Also, at the age of 45, he is only "just coming round to London", he says in a tone that strongly suggests this acceptance of the city is, at most, at the mid-point stage.
She was particularly fine as Paulina in a Brooklyn production of The Winter's Tale, savagely clawing Leontes's attendants and at one point staging a sitdown protest in his bedroom.
People rated their level of engagement for each activity on a 5-point Stage of Change scale [ 112, 113].
Correlation coefficients for the three biological replicates assessed per sample genotype and time point (stage) ranged from 0.9809 to 0.9982 after normalization.
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