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The coded values set for glucose (A) and sucrose (B) at three levels were −1 (0:1), 0 (0.75:1) and 1 (1.5:1), resulting in four factorial points (consisting of all possible combinations of the maximum and minimum levels), four axial points (one of the factors set at the midpoint) and five centre points (replicated experimental runs at the factors midpoint), all shown in Table 1.
The design used in this experiment was a 2-level, 3-factor factorial with a center point (replicated 6 times) and six axial points (replicated 2 times), with the axial points specified to maintain full rotatability.
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A total of 13 experiments including 5 center point replicates with specific anammox activity as a response were carried out.
At this point, replicates from the initial six were combined to create three replicates for the final read-out.
The other advantage of conducting center point replicates in a factorial design at 2 levels is the possibility to evaluate the presence of a curvature.
Then, for each time point, replicate and node a measurement x itr was sampled from two Gaussian distributions, either from x itr ∼ 𝒩(1200, 400) if γ* itr = 0 or from x itr ∼ 𝒩(2000, 1000) if γ* itr = 1.
Previous attempts at analyzing data from HTS experiments have been limited as they could not simultaneously include data at intermediate time points, replicate experiments and sources of uncertainty specific to HTS such as sequencing depth.
Each group and time point was replicated three times.
Each treatment and time-point was replicated three times.
Each treatment/time point was replicated three times using adults from different cohorts.
For each experiment, each data point was replicated at least three times.
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