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This effectively reduces the touch points per time resolution to under 50 times per second.
Functional images were acquired with an EPI sequence including an integrated Parallel Acquisition Technique (105 time points per time series; TR = 3 s; TE = 37 ms; 128 × 128 matrix; 1.4 × 1.4 mm in-plane voxel size; 20 slices approximately perpendicular to the calcarine sulcus covering the entire occipital and occipitotemporal cortex with slice thickness 2 mm and inter-slice gap 0.4 mm).
Functional images were acquired with an ascending interleaved echo-planar imaging (EPI) pulse sequence (90 time points per time series; TR = 3 s; TE = 30 ms; flip angle 90°; 3 mm isotropic voxels; field of view 192×192×144 mm3; 48 slices covering the entire cerebral cortex).
A time series of length 2 indicates a total data size of 20 time points per gene, i.e. 2 data points per time series × 10 TF KO experiments.
Time courses of gene expression were performed in triplicate, over 27 h, at 3 h intervals (nine time points per time course).
Significant downward trend effects with time period (T1 to T4) were found in men and women: on average 4.3 points per time period in men (95% CI –7.9 to –0.7, p=0.018) and 4.3 points per time period in women (95% CI –6.5 to –2.1, p<0.0005).
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Ninety-eight mice were used to monitor the in vivo infection of the 3 chosen parasite strains at 7 time points: four mice per time point per strain were intraperitoneally injected with 107 parasites of 2nd to 3rd day stationary phase cultures diluted in 100 µl PBS and 2 control mice per time point were intraperitoneally injected with 100 µl PBS.
At indicated time points (2 3 mice per time point), mice were sacrificed, and organs were harvested and flash frozen in OCT compound.
The percentage of cells that had undergone one or two meiotic divisions was determined at the indicated time points (n = 100 per time point).
The expression level was measured at eight time-points, with three replicates per time point at days -1, 0, 0.25, 1, 2, 3, 4, 5 post induction.
We collected multiple ants (6-10) at each time point and sampled three individuals per time point per colony for RNA extraction.
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