After quantifying the movement in the 'awake' animals, the time points of movements occurring above a threshold were detected for each of these body parts (see Materials and Methods; supplementary material Fig. S1), and were compared with the movements detected in the TI state using the same threshold.
Crucially within the Comoros island chain it was very clear that the main islands in this archipelago, Grande Comore and Mayotte, are both the primary conduits through which viruses from Africa gain access to the Comoros, and the main starting points of movements throughout the archipelago.
Due to the small size of the Charpy machine, the task of catching the reversing hammer, after the first impact at the high point of movement, was done manually by the user.
The direction of movement was determined by drawing a line from the point of inoculation to the end point of movement, and measuring the angle relative to the direction of the illumination.
Mean endpoint forces for each subject at the mid-point of movement (the 50 ms stiffness estimation time interval) were examined to determine if subjects adapted to the force fields with or without vision using different endpoint forces.
The reading on plurimeter was registered at the end point of movement i.e. when the pain was unbearable or the arm could not be moved further.
As subjects were prompted to press the right or left button as fast as possible, they were restricted with regard to the time point of movement execution, but free in terms of movement lateralization.
Spenciner and colleages [ 8] defined the borders of the neutral zone as the intersection of the y-axis (zero load) and the tangent line to the load-deflection curve drawn at the point of movement reversal (maximum load).
Since pain and stiffness pose particular problems while measuring PROM, for example in finding out the exact end point of movement, measurement of AROM could have been a good supplement.
These included percent motility, as well as the average curvilinear velocity (VCL; point-to-point velocity per sec), straight line velocity (VSL; velocity measured along a straight line from the first point of movement to the point furthest from the origin), and smooth path velocity (VAP; point-to-point velocity based upon the average path) for each video.
