Sentence examples for points for mRNA analysis from inspiring English sources

The time points for mRNA analysis were chosen on the basis of previously published observations.

At each time-point, supernatants were collected and frozen and cells harvested for mRNA analysis.

TaqMan Reverse Transcription and RT-PCR reagents were used for mRNA analysis (Applied Biosystems, Foster City, USA).

Primers for mRNA analysis are shown in Table 1.

Liver samples from 7 patients were available for mRNA analysis.

For mRNA analysis cDNA was randomly primed from 1 μg of total RNA following DNase 1 treatment (New England Labs) using M-MLV reverse transcriptase RNase H minus, point mutant (Promega) and Random primer 6 (New England Labs).

Then the cells were harvested for subsequent mRNA analysis.

The choice of 18 weeks post-ME7 inoculation as the point for systemic challenge for mRNA transcriptional analysis was based on our previous finding that robust priming of microglia occurs at this time (Cunningham et al., 2005a).

The kidney, lung, and heart were harvested and either preserved at −80°C until use for the cytokine mRNA analysis or preserved in formalin until the histologic examination.

When indicated, conditioned culture supernatants or cell layers were isolated for protein or mRNA analysis.

The cells were then lysed for protein or mRNA analysis.