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Points for expression and percentage of positive cells were added and an overall score between 0 and 6 was assigned.
In a previous transcriptomic study (Schweiger et al. 2013) we used very early and late time points for expression analysis (8, 24, and 72 hpi).
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It is a loyalty program where membership is free and fans are awarded points for "expressions of fandom".
They also highlighted that questioning could serve as heuristic devices to stimulate dialectical thinking during argumentation; act as starting points for expressions of doubts, rebuttals and counterarguments.
Data were averaged for each animal or hemisegment to produce one data point for expression and structural analyses, respectively.
Another model involves a largely environmental influence on growth but genetic variation in a developmental switch point for expression of smoltification (e.g., Aubin-Horth et al. 2006).
Inputs for the heatmaps included (1) normalized GRO-seq signals (median-centered and scaled relative to the 0 min time point for expression analyses; e.g. Figure 3B) and (2) NESs (e.g., Figure 7A).
This indicates that mRNA production is probably the primary control point for expression of AGR2 and is consistent with the mRNA-based subtractive hybridisation approach used to first identify AGR2 as a potential marker of aggressive ER α-positive tumours (Liu et al, 2005).
In this case the use of short and long term is referred to the time required for the development of atrophy condition rather to the time points used for expression profiling in the experimental design.
To use quantified gene expression patterns in dynamic simulations, reliable time points for gene expression patterns are required.
Therefore, QMb-ts myoblasts induced to differentiate at 41°C or myotubes subjected to Src activation were analyzed at different time points for the expression of p27 protein and mRNA, and for the expression of miR-221/222.
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