ADM use was defined as a time-dependent categorical variable up to each time point evaluated with three levels: never used, used intermittently (at least once but not always), and used continuously (at all visits).
As expected from the PCA, DEGs were identified between WT and Scnn1b-Tg macrophages at each time point evaluated, with 432, 394, 166, and 437 DEGs at PND 0, 3, 10, and 42, respectively, using the established significance threshold (FC ≥ 2; FDR ≤ 0.05).
To evaluate the relevance of those testing initiatives for end points associated with breast cancer, we compared the biological processes associated with breast cancer (Table 1) with end points evaluated by assays in ToxCast, EDSP, and Tox21 (see Supplemental Material, Table S1).
The sound pressure level and phase differences between two points are evaluated with the theoretical model and compared with experimental values.
A complete list of somatic mutations and allelic fractions for each clone is provided in Supplementary Table 2 and Supplementary Figs 1 and 2. Three distinct tissues from two time points were evaluated with WES (Fig. 4c).
Moreover, the concept of characteristic velocity models at flooding points is evaluated with respect to the variation of pressure drop along the column at different operational conditions.
Safety end points were evaluated with the safety analysis set, comprising all participants exposed to treatment.
Differences between values at two different time points were evaluated with a pairwise sign-rank Wilcoxon's test.
