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Cyanine-labelled cRNA (Perkin Elmer Life Sciences, Waltham, Massachusetts) was produced from individual liver samples (2 µg) from 40 mice (two time points, comprised of 4 treatment groups with 5 mice each) and URR using Low RNA Input Fluorescent Amplification kits (Agilent Technologies) according to the manufacturer's instructions.
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The test points comprises of ten new sample points within the sample space.
The primary objective was to determine the proportion of patients reaching lowDAS <3.2 after 24 weeks, secondary end points comprised the proportion of patients showing a DAS28 remission (<2.6).
Additional end points comprised other parameters of glycemic control such as insulinogenic index and serum lipids.
The primary end points comprised a composite of all fatal or nonfatal cardiovascular events and all-cause mortality.
Points on other meridians, ah shi points (unclassified tender points) and Extraordinary points comprised 16%, 14%and7%7% of total (see online supplement 2).
Results were subsequently compared with surface estimates derived from random stratified point count techniques, for numbers of points comprised between 1 and 99 per m2.
For that purpose, we reduced the comparison to 6 and 30 h, because these two time points comprised the highest number of modulated genes in the young and aged group, respectively.
The sensitivity of the assay was also tested in a duplex end-point reactions comprised of a constant level of 150 copies of synthetic control-DNA and a clinical sample of spleen tissue diluted serially from 10−1 to 10−5.
Jadad is a 5-point scale comprised of items related to sequence generation, blinding, and withdrawals.
Each decay profile for each time point was comprised of 400,000 recorded photons.
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