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We use a first order segmentation to extract candidate feature points and process them as a graph to recover the sharp feature lines.
The AnalyzeSkeleton plugin (http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:analyzeskeleton:start) was then applied to such images to collect data on the number of branches, end points and process lengths.
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Naive neurons (15 DIV) were treated with purified exosomes for the indicated time points and processed for immunocytochemistry.
Companies that follow this practice maintain tight, resourceful operations throughout their early stages and only add capital when there are proof points and processes to support it.
In practice, each contour, denoted, is sampled with (2D) points and processed in order to extract the underlying convexity/concavity coefficients at different scales [2].
Samples were taken at the indicated time points and processed for immunoprecipitation with Flag beads (Sigma-Aldrich), followed by SDS-PAGE and autoradiography.
Grafts were harvested at different time points and processed for histology, immunocytochemistry or electron microscopy.
Cells were further incubated for indicated time points and processed for western blotting.
Epithelial cells were harvested at different time points and processed as previously described.
Clinical data as well as tissue and serum was obtained at various time points and processed according to established protocols.
Differentiated cells at 5 × 104 cells/ml were fixed at different time points, and processed for immunostaining and evaluation of differentiation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com