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For gravimetric determination of dry cell mass 10 ml of the culture broth from each sampling point was transferred into dry-weighed 15 ml sampling tubes and centrifuged for 20 min at 4643×g and 4 °C.
The paper point was transferred into 200 μl G2 buffer of the EZ1 DNA Tissue Kit (Qiagen, Hilden, Germany) and stored at −20°C until transportation to the Institute of Medical Microbiology and Hospital Hygiene at the Heinrich Heine University for analysis.
This time point was transferred manually to Interact 3.1.
Thereafter the position of this point was transferred to all other subsequent examinations of the same shoulder, using its computed position relative to the humeral head markers.
The paper point was transferred into 200 μl of phosphate-buffered saline (PBS) and centrifuged at 12,000 × g for 5 min. After centrifugation, the bacteria chromosomal DNA was extracted using MORA-extract (Cosmo Bio, Tokyo, Japan), according to the manufacturer's instructions, and then served as a template.
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Over a period of 23 days, medium that was incubated with the rhBMP-2-releasing PlnD1-conjugated PCL constructs (or unmodified PCL scaffolds) for 1 day (for the first two time-points) or 3 days (for the rest of the time-points) was transferred to fresh W20 17 cell cultures at designated time-points to determine the levels of alkaline phosphatase activity.
The points are transferred into the app's wallet, and can then be used to buy books.
The test points were transferred into a culture medium including the GP-point material with a concentration of 6 mg/ml, and eluates were obtained after 72 h.
The paper points were transferred into 750 μl of sterile Ringer's solution.
Cultures from different survey points were transferred to the National Tuberculosis Reference Laboratory by air.
If that happens before the gained points are transferred to the permanent bank, all points for that balloon are lost.
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