Sentence examples for point substitution from inspiring English sources
Deletion and point substitution mutant clones were generated using a QuikChange Site-Directed Mutagenesis Kit (Agilent, Clara, CA).
The point substitution was different but occurred in the exact same spot and interfered with the drug in the same way.
The edge support conditions of the plate are satisfied by forcing the boundary points to meet the geometric boundary conditions of the plate via a point substitution technique.
We also identified a substitution within the binding pocket (Q266I) that cooperates with G230I and the previously identified S162A binding-pocket point substitution, rendering hSTING highly sensitive to DMXAA.
Consequently, SInv and 5 mutants including three mutants with single point substitution [Mut1 = P152 V, Mut2 = S85 V and Mut3 = K153F ], one mutant with two points [Mut4 = S305V-N463 V] and one mutant with three points [Mut5 = S85V-K153F-T271 V] were developed via site-directed mutagenesis and produced using Pichia pastoris as the host.
Four mechanisms are largely responsible for the creation of new MHC alleles: point substitution, allele conversion, gene conversion, and recombination.
The α1c-D contained four point substitutions at amino acid residues known to be critical for dihydropyridine binding.
Finally, the possibility to achieve sequential multiple point substitutions via the Sonogashira cross-coupling method, during solid phase peptide synthesis, was also evaluated.
Second, the analysis of point substitutions in TBEV E protein ectodomain in MD simulation study revealed the basis of their influence on the virion properties.
A mutant with three point substitutions, Lys175Met/Cys176Gly/Tyr273Leu, i.e., HaloTag, has a high apparent second-order rate constant, thus allowing the labeling reaction to reach completion even under low haloalkane ligand concentrations [260].
Two types of somatic mutations (in-frame deletions and point substitutions) in the EGFR gene were successfully identified within 3.5 h using this system, suggesting that this system could be used in clinical tests of EGFR gene mutations in lung cancer, and potentially other cancer, patients.
