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OCT is a point scanning system based on the use of a NIR source coupled to a Michelson interferometer.
Each region was imaged separately using an Olympus FV1000 point scanning confocal microscope.
Microscopy was performed on a single photon point scanning confocal Olympus Fluoview 1000 microscope (Olympus).
Images were captured by Zeiss LSM 510 META point scanning laser confocal microscope.
For comparison, these values are plotted with a theoretical model of a point scanning system [ 22] in Fig. 5 to demonstrate the sectioning capability of the line-scanner with respect to the standard confocal microscope.
For instance, there is temporal wavefront instability in the form of micro-fluctuations of accommodation [ 16, 30], tear films, a point scanning system aberration, involuntary eye motion, and some other factors.
Similar(27)
The measurement is based on a point-by-point scanning so values of the four measured functions are linked in space and in time.
Point-scanning was realized with a pair of high-speed galvanometric mirrors (Lightning DS, Cambridge Technology).
Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy.
Several advances in 3D fluorescence microscopy now offer higher resolution, improved speed, and reduced photobleaching relative to traditional point-scanning microscopy methods.
By using a wide-field epi-fluorescence arrangement instead of point-scanning, the system complexity and cost are greatly reduced.
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