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The design used in this experiment was a 2-level, 3-factor factorial with a center point (replicated 6 times) and six axial points (replicated 2 times), with the axial points specified to maintain full rotatability.
Thus we designed a new set of sfGFP BiFC partners (Gn, Gc), with the split point replicated from previous studies using overlapping BiFC YFP fragments [24,26,27,50] — Gn contained β-strands 1 8 of sfGFP (2 172), and Gc β-strands 8 11, sfGFP (155 238).
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A total of 13 experiments including 5 center point replicates with specific anammox activity as a response were carried out.
At this point, replicates from the initial six were combined to create three replicates for the final read-out.
The other advantage of conducting center point replicates in a factorial design at 2 levels is the possibility to evaluate the presence of a curvature.
Then, for each time point, replicate and node a measurement x itr was sampled from two Gaussian distributions, either from x itr ∼ 𝒩(1200, 400) if γ* itr = 0 or from x itr ∼ 𝒩(2000, 1000) if γ* itr = 1.
Three LC parameters have been optimized using a multifactorial design comprising 29 experiments that includes 2 center point replicates.
The factors in the 23 factorial experimental design with a center point replicate included pressure (83.7 and 97.5 bar), solution concentration (15 and 30 mg/mL), and solution flow rate (2 and 4 mL/min).
The center point replicates were chosen to verify any change in the estimation procedure as a measure of precision property.
Another important result is that the experimental error is only (2.5 %) when considering the center point replicates.
This cubic design is given by a set of points at the midpoint of each edge of multidimensional cube and a center point replicate (Palamakula et al. 2004).
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