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Its appearance for a short period of time suggests that conidia undergo major osmotic changes particularly at this time point of germination.
Trees were destructively sampled to collect approximately 20 80 mm thick disks, or cross-sections, from individual trees at 0.3 m from the point of germination.
For example, S3 stage marked the end point of germination process, and can be easily identified by the visible coleorhiza emergence from the grains, which typically occurred at 18 hours of germination.
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Os04g0616400 encoded for a protein similar to a receptor-like serine/threonine kinase and was more expressed in Selenio, with respect to Dorella, in both treatments and time-points of germination (log2FCs ranging from 5.37 to 66).
Germination curves were fitted using the four-parameter logistic function of SigmaPlot v.9 software to calculate the mid-time point of the germination curve (T1/2).
By contrast, most of the diminished genes were detected at the onset of germination between time points t1 and t2.
Most of the sigma factors were activated in the first phase of germination between time points t1-t2 (the initial 10 min activation period) and t2-t3 (0-30 min of cultivation), including the known sigma factor SigR, SigE, HrdD, SigD and SigH.
All pair-wise comparisons between genotypes are shown in Table 1, for time point "out of steep", and Table 2, for "3d of germination".
SEM imaging also showed the point of invasion of bacteria into spores at the place of germination (Fig. 2b,c,d).
At each time point, the number of budding cells was counted as a measure of germination.
For simplicity, throughout the paper we refer to the analysed time points T1, T50 and Tmax as different 'germination stages' (beginning, progress and end of germination, respectively) though keeping in mind that Tmax can be also referred as 'seedling establishment' since at this time point the seeds have left the germination processes already.
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