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Furthermore, such sporadically duplicated genes are significantly enriched (Hyper-geometric test, p<0.05,) among all the reassigned genes (duplicated or not) at their point of duplication, but not at later phylogenetic points, when they behave comparably to non-duplicated genes.
Gene duplication events significantly contribute to regulatory divergence, in particular close to their phylogenetic point of duplication.
For genes with duplications, we considered reassignments only at and after the point of duplication, and handled the paralogous copies as two data samples.
To assess the extent of regulatory neo-functionalization of targets, we compared the module assignment of the two paralogs at the point of duplication to that of their immediate pre-duplication ancestor (four possible fates, Figure 10C, 1 4).
Each row corresponds to a GO process (column 1), and the subsequent columns indicate the phylogenetic point of duplication and numbers used to estimate the p value from the Hyper-geometric test.
Further, not only is the exponential loss model exclusively consistent with a constant neutral rate of loss but it also decays to zero (where all duplicates are lost) and is not defined at t = 0, the point of duplication.
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Hence, the H. arabidopsidis genome encodes a unique repertoire of expanded OGs, while at the same time, ancestral OGs, that is, OGs that were already gained before the point of duplications, were either completely lost or contracted in size.
To further delineate the precise point of gene duplication and to trace gene duplication in spite of subsequent gene losses, we applied phylogenetic timing to all gene families, like we did for the 2HOM blocks.
Bayesian, ML and NJ analyses placed the point of MyoD1/MyoD2 duplication as a specific event within the Acanthopterygii when a gamma distribution of among site rate variation was used which is known to be resistant to LBA [17] (Fig. 1a c).
In these cases, the sequenced fragment was hypothesized to span one break point of the duplication.
In most cases, divergence in paralog assignment is already apparent at the immediate phylogenetic point of the duplication event (at the phylogenetic resolution of this study).
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