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Sequence and MALDI-MS analyses were used in conjunction with a fluorometric assay to determine the exact point of cleavage by each MMP.
The precise point of cleavage of the linker peptide when expressed in rice plant cells remains to be determined.
As recently reported, the dichotomy in the grouping can be explained by a region of significant diversity, encompassing the C-terminus of α43 and the N-terminus of β43, overlapping the point of cleavage between the two domains.
At the final stages of DNA binding and cleavage (Figure 5C), the quinolone resistance sites (Ser 79 and Asp 83, which are located within the helix α4 bound to the minor groove of the DNA molecule) will be positioned next to the 3'-end of the cleaved DNA strand with Ser 79 being closest to the point of cleavage (Figure 5C).
All three converge at the point of cleavage of C3.
Depending on the exact point of cleavage by γ-secretase, three principal forms of Aβ, comprising 38, 40 or 42 amino acid residues, respectively, are produced.
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Comparison of residues at the P5 position of the five points of cleavage within the natural substrate, Asp, Glu (×2), Ser, and Lys, reveals a diversity of residue types (10).
Comparison of the five points of cleavage in the ORF 1 polyprotein reveals that the residues at the P2 position always possess a hydrophobic side chain: Leu (×3), Met, and Phe (10).
The substrate specificity of Autophagins has been partially determined, with glycine required at the point of hydrolytic cleavage [29].
To ensure characterization of the appropriate epigenetic context, we used the apoptotic end point of PARP cleavage to identify tolerated and toxic exposures of As2O3 in both cell lines.
While the RCL sequence of chicken Serpinb10a and human SERPINB10 have some similarities, there are a number of charge differences throughout the RCL, and optimal alignment requires insertion of a gap at the P side (N-terminal to the point of proteolytic cleavage), a feature not observed between other chicken-human orthologues.
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