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He fought against excessive spending, to the point of being prepared to see the federal government shut down.
Fashion has embraced the all-in-one, even to the point of being prepared to forgive the extra time they necessitate in the loo.
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Models of receptor point mutants were prepared by side chain substitution in previously prepared homology models and then subjected to energy minimization prior to ligand docking.
To test the functional relevance of the candidate transcription factor binding sites, point mutations were prepared to inactivate each of the identified conserved motifs in the context of the −219/+114 FSCN1 luciferase reporter plasmid (Fig. 4A, Fig. 5A).
To determine the catalytic role of this residue, various point mutants were prepared and characterized.
The standard normal point algorithm was prepared before the effects of the satellite signature were fully recognized.
[The progression continues] through the run of the stories to "The Final Problem," at the end of the "Memoirs," at which point he's prepared to lay down his life to stop Moriarty.
In the present study, base map showing locations of investigating points has been prepared using Survey of India (SoI) Topo sheets 73 I/15, 73 I/16, 73 M/3 and 73 M/4 and satellite imagery (IRS-IB, LISS-II).
Standard curve was consisting of a 10-fold serial dilution series of five points which was prepared from cDNA samples (35 ng/μl) and each dilution was tested under a range of temperatures around the calculated Tm of the primers used in the experiments; untranscribed RNA samples were used as negative controls.
For each time point duplicates were prepared.
Additionally, they present important advantages: from a safety point of view LNs are prepared with well tolerated components, and from a technological point of view, they can be easily produced at large-scale, can be subjected to sterilization and lyophilization, and have shown good storage stability.
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