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As part of this work four manual signal boxes were replaced by three power signal boxes, and the semaphore signals and mechanical point linkages were replaced by colour light signals and point motors.
In this way, linkage groups were created between markers that were linked with a two-point LOD score > 5, had a minimum of 0.4 times the average number of meioses, shared linkages with no more than six other groups and had a minimum linkage ratio (i.e. the proportion of two point linkages for a given marker to other markers in the same linkage group) of 0.5.
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The average calibration constants for the rear wheel speed, fuel consumption and three point linkage transducers were 0.0364 m/pulse, 0.000142857 l/pulse and 0.66 mV/kN respectively.
Two point linkage identified 6 regions with LOD scores >3.0 (Fig. 5a).
Simulated two point linkage analysis predicts a maximum logarithm of the odds (LOD) score for the family of 3.17 at Θ = 0.00.
Two point linkage analysis was carried out using the MLINK routine of FASTLINK v4.1P [26], [26] with a dominant transmission model: Penetrance of 0.95, phenocopy of 0.001, and the disease allele frequency of 0.001.
b Two point linkage recombination between markers from the two most distant clones in the contig.
c Additional markers that were previously isolated from other BACs in the contigs were linked to the new BAC end sequence microsatellites by two point linkage analysis and included in this table.
We validated single-point linkages using a SNP microarray containing 328,125 SNPs.
Two-point linkages for thirty other EST-based SNPs, selected to refine the comparison of bovine chromosome (BTA) 19 with human chromosome 17, were also obtained [ 11].
This paper analyses three different types of reference conditions, namely: free linkage, rigid linkage and two-point linkage.
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