Linkage of the gained information with quantitative trait loci (QTL) studies for iron efficiency related traits in the IBM population (Benke et al., unpublished) allows to pin-point differentially regulated genes that co-localize with QTL confidence intervals and thereby represent excellent candidate genes underlying this trait.
The signalling downstream of receptors however is likely to diverge at some point to differentially drive different secretory events.
The mapped reads point to differentially methylated areas across the genome, with some marked preferences for hypermethylation or hypomethylation in certain genomic regions.
For each time point, the differentially expressed genes from the Qiagen-NRSP8 microarray were classified into biological processes using GO terms when available (Table 3).
As seen in Fig. 7d, e, many genes that do not show proximal GR binding at the 1-hour ChIP-Seq time point are differentially expressed at the 8-hour microarray time point.
In high phenols samples, isoelectric point of differentially expressed proteins was comprised between p I 5.22 and p I 8.64, with an apparent molecular weight from 13306 Da to 95563 Da (Table 1).
Means of log-fold change values for each time point of differentially expressed genes were hierarchically clustered using Cluster 3.0 (Eisen et al, 1998) to identify genes with similar temporal profiles.
By comparing six consecutive time points, 3445 differentially expressed genes were identified.
Red points are differentially expressed genes (Log2RPKM>1, p-value<0.05, Log2 FC >1.4).
Blue points are differentially expressed genes (Log2RPKM>1, p-value<0.05, Log2 FC >1.4).
By comparing 11 consecutive time-points, 9284 differentially expressed genes were identified and annotated during this study.
