As the reviewers pointed out, dot blots are not ideal, as the high concentration of protein can lead to non-specific binding of antibodies and, because the proteins are not separated by size, it is not possible to see whether the source of the signal is from one protein or multiple proteins.
"My father liked to entertain and have houseguests, but he was also a very private person, and we were, too -- at some point," Ms. Blot said.
Total RNA samples were prepared at the time points indicated and analyzed by dot blotting.
There was no point in blotting out the winner's publicity with a storm of "Beryl Bridesmaid Again" headlines.
Tissue iron content, HAMP mRNA levels (96 h time point), western blotting and HPLC: data were analyzed using 1-way ANOVA, with maternal treatment (SAL-VEH vs. TURP-VEH vSAL-IRONRON vs. TURP-IRON) as between-groups variable and furthered with Fisher's LSD post hoc tests when ANOVA was significant.
Following treatment with indicated concentrations of drug for specified time points, Western blot was performed as described previously [ 36].
Bone marrow-derived macrophages, generated as described previously, were stimulated with 200 ng/ml LPS (026 B6; Sigma) harvested at the indicated time points and blots were probed anti-p-p65 (Ser536), p-I κB α (Ser32), I κB α, p-ERK, ERK, actin (Cell Signaling Technology, Frankfurt, Germany) and p65 (Santa Cruz Biotechnology, Heidelberg, Germany).
Figure 4 shows the gene copy number delivered in two separate rabbit corneas detected at 2-week time point using slot blot method.
Control sera from 10 other volunteers (N5~N14) (7 males, 3 females; average age, 42.0 years) were also collected at one time point for Western blot analysis.
In addition, we assessed the cytosolic levels of cytochrome C and NF-κB at the 24-hour time point using western blot analysis.
