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The precision, efficiency, false positive rate, limit of detection (LOD95%) and probability of detection (POD) were determined, and the results demonstrated that both bovine and ovine detection methods performed well.
Activities of POD were determined by the method of Chance and Maehly [ 18] with some modifications.
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Activity of POD was determined based on the increase in absorbance read at 470 nm, and one unit of POD activity was defined as the increase of absorbance by 0.01 per min. CAT activity was measured by the depletion of H2O2 at 240 nm [ 53].
The activity of peroxidase (POD) was determined as described by Jiang et al. (2002).
The effective number of pollen donors per pod was determined as Ne = 1/ rppm.
The activity of POD was determined as described by Zhang et al. [ 52].
The approach includes the following three features: As explained in Science & Decisions NRCC 2008, box 5 3), a median human POD, accompanied by a lower bound for the POD, is determined from chemical-specific animal bioassay data and (usually) nonchemical-specific data on differences in animal and human sensitivity.
Resting motor threshold (MT) in M1, defined as the lowest intensity of TMS which yielded motor-evoked potentials (MEPs) of at least 50 μV in 5 out of 10 trials using an MEP pod, was determined from electromyographic (EMG) activity in the abductor pollicis brevis using surface electrodes.
POD activities were determined specifically at 420 nm.
POD activities were determined as described by Quiroga [ 74].
The contents of Ca2+ (in leaves) and Cd2+ (in leaves, pods, and seeds) were determined using a Perkin-Elmer Model 3300 Atomic Absorption Spectrophotometer (Chapman and Pratt 1961).
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