Electrolytic leakage, the contents of chlorophyll, MDA, and H2O2, and the enzyme activities of SOD, CAT, and POD were measured.
As ROS-scavenging enzymes play critical role in detoxifying ROS activities of three key enzymes, superoxide (SOD), catalase (CAT) and peroxidase (POD), were measured in the two transgenic lines and WT before and 7 d after drought.
After drying of harvested pods under natural conditions for two weeks, the length, thickness, width, and weight of the matured pods were measured.
At harvest (approximately 120 d after planting), peg length (PL) and pod constriction (PC) was measured with six replications.
The number of seeds per pod (SDNPPD) was measured in ten pods.
Pod dehiscence was measured either by direct observation of the pods on the plant or by drying freshly harvested pods in an incubator (Weeden et al., 2002).
POD activity was measured by the increase in absorbance at 470 nm due to guaiacol oxidation.
Superoxide dismutase (SOD) activity was assayed following the method from Giannopolitis and Ries (1977), while peroxidase (POD) activity was measured as described by Lee and Lin (1995).
POD activity was measured using modification of the procedure of Egley et al. ([1983]), the reaction mixture in a total volume of 2 mL contained 25 mM (pH 7.0) sodium phosphate buffer, 0.1 mM EDTA, 0.05% guaiacol (2-ethoxyphenol), 1.0 mM H2O2 and 100 μL enzymes extract.
POD activity was measured spectrophotometrically by the method of Khan and Ahmed [ 15].
