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At 2∼3 weeks, the culture reached an optical density (OD600) of 0.6∼1.0 (approximately 1×10 to 1×10 colony-forming units (CFU /mL) and was plated onto 7H11 agar plates containing 100 to 500 µg/mL POA (pH 6.8) or 25 to 200 µg/mL POA (pH 5.7) and also plated on a 7H11 plate for CFU counting.

Conversely, genes that reacted significantly different to either 10 μM 20E, PoA or 0.5 μM 20E were enriched in many unique categories; the most strongly enriched functional terms are associated with genes down-regulated by PoA relative to 0.5 μM 20E (Table 3).

Hybridization is also commonly reported within the genus Poa [ 27, 28, 63- 65] and the entire grass family [ 9].

Following the same arguments as in the case of the game G ( a ), we state that a unitary PoA (PoA=1) is observed only when the set of NE is unitary and transmitters do not share the same channels to transmit.

The graphs show that when comparing case 1 with case 2, there is a shift of plan towards the city outskirts, because both PoA 153 and PoA 178 are least congested due to their locations.

For example, PoA 33 reside in region 33, which is shown at 34th row on two graphs.

The PoA [37] and the PoSa[38] are both measures of the efficiency of the set of equilibria of a game.

In Figure 7, we plot the PoA (29) of both games G ( a ) and G ( b ) as a function of the number of transmitters for several SNR values.

Case 1 The agents after visiting the PoAss labeled, 6 and 33, start shifting towards the PoAss numbered, 54 (more) and 63 (less), followed by PoA 63 and others.

Table 2 Simulation parameters Parameter Value Parameter Value Speed 1 m/s Mobility model Rectangle # of POA 2 Packet flow Bi-dir CBR # of MN 1 UDP packet transmit rate 0.13 s AP power 2.0 mW Beacon freq.

It shows  PoA-wise details of all the cases considered in this work and described below: 1. Case 1 The agents after visiting the PoAss labeled, 6 and 33, start shifting towards the PoAss numbered, 54 (more) and 63 (less), followed by PoA 63 and others.

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