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Using the Vitis GeneChip, Fung et al. reported that 625 transcripts in V. vinifera and only three transcripts in V. aestivalis were involved in host responses to PM. Fekete et al. identified 25 PM-responsive genes in V. vinifera (cv. Cabernet Sauvignon) that were upregulated specifically in response to E. necator.
A similar number of PM-responsive genes was detected in 'Carignan' at both time points.
PM-responsive genes specifically associated with the susceptible phenotype were also detected.
Thirty-five PM-responsive genes were discovered with similar patterns of differential regulation in 'Karadzhandal', O34-16, anDVIT3351.2727 and opposite trends in 'Carignan'.
Expression of the seven PM-responsive genes located in this region was confirmed by qRT-PCR (94.64% validation for 56 comparisons; Figure 5e).
Transcriptome profiling revealed a set of 51 PM-responsive genes shared by all Central Asian accessions, which may be the signature of a common Ren1-dependent response (listed in Table 2).
Investigation of the PM-responsive transcriptome of the two grapevine species revealed that an ortholog of the Arabidopsis Enhanced Disease Susceptibility 1 (EDS1) was differentially expressed in Norton and Cabernet Sauvignon.
Common PM-responsive genes associated with partial resistance were identified by applying a double fold-change cutoff: common up-regulated genes were genes with log2FC ≥ 0.5 in at least six Central Asian accessions and log2FC < 0.01 in 'Carignan'; common down-regulated genes were genes with log2FC ≤ −0.5 in at least six Central Asian accessions and log2FC ≥ −0.01 in 'Carignan'.
Since orthologs often maintain the same function during evolution, we conjecture that the expression of these genes might also be responsive to PM fungi attacking corresponding species.
It is somewhat surprising that many studies showed that these susceptible populations seem less responsive to PM exposure compared to healthy individuals.
On average 61.0 ± 20.3% of the genes previously reported to be responsive to PM infections were found differentially expressed in our study (Supplementary Dataset S6).
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