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Another advantage lies in the use of an FDML laser with a PM cavity and a PM buffer stage.
In other words, what is the pM buffer capacity of the system?
Transfer the solution to a dialysis bag and dialyze against PM buffer.
It is equipped with a PM buffer stage that doubles the fundamental sweep frequency of 54.5 kHz.
Dissolve the pellet in a minimal volume of PM buffer and dialyze against polymix with 3 dialyze changes.
Collect the precipitate by centrifugation at 4000 g for 20 min (4°C) and dissolve the pellet in 2 ml Polymix (PM) buffer.
Similar(50)
Sequencing libraries were denatured with sodium hydroxide and diluted to 3.5 pM hybridization buffer before loading into a lane of an Illumina GA flowcell.
Biotinylated F1 (500 pM) in buffer A was infused into an observation chamber which was constructed of two coverslips: a bottom one (24 × 32 mm) modified with Ni-NTA (9) and an untreated top one (18 × 18 mm).
Briefly, crude extracts from organs were prepared homogenizing with polytron in PM-2 buffer containing 33 mM NaH2PO4, 66 mM Na2HPO4, 0.1 mM MnCl2, 2 mM MgSO4, 40 mM β-mercaptoethanol, pH 7.3 and centrifuging twice for 10 min in a microfuge, discarding the pellet and lipid layer.
The heterogeneous equilibrium causes the specificity of these buffers with respect to mono-phase buffers; the pM at which the buffer capacity is maximum, namely the pM range of the buffer action, can be changed by varying the amount of the second phase.
PCR amplifications were performed in a total volume of 25 μL containing 20 ng genomic DNA template, 1 × PCR buffer, 20 pM each primer set, 0.2 mM each dNTP, 1 U Taq DNA polymerase (VIVAGEN, Korea).
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