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They understand that the politics of malnutrition are too important to be dissolved in a Diet Coke Plus solution.
He told me: "You need a 'Barnett Plus' solution for Wales.
The heat-induced antigen unmasking was performed in Citra Plus Solution, pH 6.0 (BioGenex, San Ramon, CA) for 5-10 minusingusing an autoclave oven.
This arises because it is necessary to have a certain concentration of nitrogen in the available soil pool (freely-exchangeable plus solution nitrogen) from which the crop can take up the nitrogen it needs, and concurrently the available soil pool will lose nitrogen at a rate controlled by various biophysical processes.
Twenty μL volume of quantitative PCR reactions comprised SYBR Green Realtime PCR Master Mix-Plus (10 μL) (TaKaRa Bio Inc., Japan), Plus Solution 2 μL (TaKaRa Bio Inc., Japan), PCR primers (1 μL of each), sterile water (4 μL), DNA sample (2 μL).
Membranes were stripped of antibodies for 30 min (Re-blot plus solution; Millipore), rinsed, and blocked at room temperature for 2 h.
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He has moved a good way from his first defiance, agreeing in principle to some kind of UNSCOM-plus solution (though not, as yet, an acceptable one) but insisting on a two-month deadline, which is also unacceptable.
CFTR was detected by a horseradish peroxidase-coupled secondary antibody (AbCys, Paris, France) and incubation with the ECL-Plus solution (Amersham, Little Chalfont, UK).
As previously described [15], total RNA was prepared from diencephalic brain samples using a RNA-plus solution (Q-Biogen, Illkirch, France).
Normal human BM mononuclear cells were separated from BM aspirates (AllCells) using Ficoll-Hypaque-Plus solution (GE Healthcare, Tokyo, Japan).
Isolated leukocytes were immediately stored at -80°C in 1 ml of RNA-PLUS solution (Qbiogene, Strasbourg, France).
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