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The half-life of the KIT protein in each group was estimated after plotting KIT protein expression relative to the untreated control.
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The catalase activity of PAA-CNPs was determined using a catalase kit and plotted as a function of WPAA/ WCNP for all molecular weight of PAA used for coating CNPs.
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The percent maximal inhibition at VEGFR-1/2/3, PDGFR- α/ β, Flt-3, and c-Kit was plotted as a function of inhibitor concentration to yield a concentration response curve.
KIT expression (RQ) plotted against the gene's copy number showed no significant correlation between the two parameters (p > 0.05, Figure 3C).
Growth curves were plotted with a Cell Counting Kit-8 (CCK-8, Dojindo) according to the manufacturer's instructions.
To quantify the effect of 1A6/DRIM depletion on cell proliferation, a cell growth curve was plotted using the Cell Count Kit-8 assay.
Standard curve was plotted using the glutathione standard supplied in the kit and used to calculate the experimental values.
The cell viability percentage was determined according to the kit protocol, and cell viability percentage was plotted on a graph.
Purified cAMP provided by the kit was diluted into different concentrations to plot the standard curve.
The absorbance values of each standard were plotted as a function of MDA concentration (Cayman's kit protocol).
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