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The rat lenses were imaged with a digital camera (Power Shot A2200, Canon, India) and analyzed by ImageJ software (http://rsbweb.nih.gov/ij/) to generate plot profiles of pixel intensities and brightness along all points on a selected line.
Open image in new window Fig. 1 Plot profiles generated by ImageJ showed cataract distribution (y-axis) relative to the distance from the center of the lens (x-axis) Open image in new window Fig. 2 Histo-architecture of eye lens tissue: viewed under 10× magnification (a c) and 40× magnification (d f).
Since all three of these immuno-stimulatory herbal extracts produced relatively similar scatter plot profiles, an extract prepared from the immuno-suppressive herb, Urtica dioica, was studied.
In addition, the skyline plot profiles for all the data sets analyzed, except for the OLC one, were in a steady non-expanding "lag" phase up to about 1880, then grew exponentially up to the 1940s, and finally were again in a lag phase after the 1960s (Figure 2 A, B and D).
The skyline plot profiles using 5×10−4 s/s/y as substitution rate showed an exponential growth of the effective number of infections for the CdE and Fr data sets for a period of about 60 years (from 1880 1890 to 1940 1950), after which it remained constant.
Dot plot profiles are representative of at least three experiments.
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Plot profile data were exported and plotted using excel to calculate the arbitrary distribution of cataract in all the group of lenses.
The plot profile analysis also indicated a minimal opacification in the rat lens treated with C-PC (Fig. 1) when compared to the Group II.
The number of axons in comparable regions was measured by plot profile analysis (ImageJ).
Hydrophobicity plot profile comparison has been used to identify tentatively these polymerases [57].
The image was then split into individual colours and the plot profile data was collected for each signal.
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