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CO2 was administered as a 1 min pulse in a continuous air flow from a compressed air tank (ambient air with ~ 385 ppm CO2), with a flow rate of 0.5 L min−1 to a single leaf inside the plexiglass leaf cuvette (internal volume 0.09 L).
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For secondary containment, a plexiglass tube (0.3 m diameter × 1.8 m length) was customized with an adjustable leaf cuvette mounted inside the plexiglass tube on metal rails and a door panel to access and manipulate the plant (Fig. 1a, b).
More plexiglass?
Plexiglass $100.
"Plexiglass was new.
It's actually mirrored plexiglass.
He hovered around the plexiglass.
Then I sawed the plexiglass into pieces.
He framed the finished product behind plexiglass.
Then leaf subsides to leaf.
"A leaf is a leaf.
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