Sentence examples similar to plex analyses from inspiring English sources

However, the correlation between Proseek and Bio-Plex analyses for PDGF-BB was particularly strong (p and q values = 0) (Additional file 1: Table S7).

We next compared Mascot ions scores of peptides detected at least once in both the iTRAQ 4-plex as well as the iTRAQ 8-plex analyses of the protein mixture, as these two reagents were found to differ most strongly with regard to peptide identification rates.

98 peptides were not detected in any of the four 8-plex analyses applying our stringent search criteria (Mascot ions score threshold 20, rank 1, Mascot significance threshold 0.05, minimum peptide length 8, maximum peptide mass deviation 8 ppm) that ensured false discovery rates less than 1% on both the peptide and protein levels in all analyses.

These observations were corroborated by similar results in the HeLa experiments, where we divided the set of 1073 peptides that were detected in two out of two technical replicates using iTRAQ 4-plex into two groups: 713 that failed to be detected in any of two technical replicates with 8-plex (4-plex-only), and 360 that were detected in either or both of these 8-plex analyses (4-plex-and-8-plex).

To study whether these cytokines might derive from moDC, we analyzed undiluted cell culture supernatants from immature and matured moDC by ELISA and 25-plex Luminex analyses.

Using 25-plex Luminex analyses, we detected significant different levels of IL-7, IL-12p40, TNFα, IFN gamma and MIP-1α MIP-1αure moDC from pSS patinnts comature with controls (Table  2).

Bio-plex data were analysed using the Bio-Plex Manager software version 3.0 (Bio-Rad Laboratories).

Analyses were performed on Bio-Plex 200 Luminex instrument using Bio-Plex software (Bio-Rad, Hercules, CA, USA).

Multiplex PCRs were performed with 144-plex VeraCode SNP arrays (Illumina) using these samples, and genotype analyses were performed using custom 96-plex SAM arrays for 96 samples.

Measurements and data analyses were performed with the Bio-Plex system in combination with the Bio-Plex Manager software 6.0 (Bio-Rad Laboratories).

Standard curves were included in all protein analyses, and optimized using Bio-Plex Manager 6.1 (Bio-Rad) for all Milliplex assays, and the Akima method for curve fitting in Magellan v.7.0 for the ELISA.