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FACs were re-recorded throughout the playback period to prevent pseudo-replication and account for potential changes in the pups' voice.
Variables measured during the playback period included the change in song rate compared to pre playback baseline levels (# songs/min), latency to approach playback loudspeaker (s), minimum approach distance (m) and time within 5 m of the loudspeaker (s).
For the post playback period we assessed the subject's time within 5 m of the loudspeaker (s) and the change in song rate compared to baseline levels (# songs/min).
Because of this experimental design both control and experimental periods varied in length (control mean = 43.1 min, range 28 109 min; experimental mean = 36.4 min, range 17 61 min; N.B. in a few instances the playback period was <30 min because the focal bird's partner was present when they visited the nest towards the end of the experimental period, so no begging calls were broadcast).
It appears that birds may have misidentified that the playback period was not yet over.
We noted the duet types sung before, during and after the playback period.
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And an independent CBS survey of viewers, conducted the first two weeks of the fall season, suggests certain nights of the week are becoming popular playback periods.
Anecdotally, we noted an increase in vocalisations during playback periods that may have had this function.
During the second and third playback periods, we broadcast the two test stimuli, with half of the subjects receiving one type of test stimulus first and the other half of the subjects receiving the other type of test stimulus first.
Each experiment followed the same scheme and lasted for 44 min in total: 5 min pre-playback period of silence and three subsequent periods each lasting 13 min consisting of 3-min playback followed by 10 min of silence each.
Six of the 22 subjects gave one or two coos in the 3-min period of playback, and one additional subject gave a coo in the 10 min following the playback.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com