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CFU numbers were determined from the liver, kidneys, and spleen 3 and 5 days after infection by plating tissue homogenates on YPD agar.
We observed that expansion of TILs was more efficient following enzymatic dissociation or plating tissue fragments (data not shown) and therefore these methods will be used for processing tissues for our planned clinical trials.
Secretion assays were conducted on cultures 1 day after plating tissue on 24-well plates.
Reisolation of App was achieved by plating tissue of each of the seven lung lobes on selective meat and blood agar [ 53].
Plague was tested for by plating tissue samples (gerbils) or pools of fleas on Hottinger's agar containing 1% haemolyzed sheep erythrocytes.
Similar(55)
As shown in Figure 4B and 4C, the outgrowth from plated tissue is established 5 days after plating with no detectable GFP fluorescence.
As shown in Figure 1A, the gross histological appearance of the outgrowth from the plated tissue of different species was morphologically similar.
After several days in culture, a layer of stromal-like cells arose from adherent plated tissue (termed cardiac explants) over which small, round, phase-bright cells migrated.
The UCMSCs were harvested and cryopreserved 20 30 days after the initial processing, as time was required for the UCMSCs to migrate from the plated tissue).
Prior to plating, the tissue culture flasks were coated with collagen for increasing the cell attachment.
Striatal tissue was dissected on an-ice-cold plate, tissue pieces weighed and snap-frozen in liquid nitrogen.
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