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The number of viable cells was determined by plating out serial dilutions on TSBA.
Two weeks after plating out on selective agar containing HmB, the protoplasts developed cell-walls and formed colonies.
Cells were enumerated by the Miles and Misra technique which involves a 10 fold dilution series in PBS followed by aseptically plating out 20 μl of each cell suspension in triplicate on appropriate media, which were then incubated anaerobically at 37°C.
The number of CFU in each inoculum was verified by plating out serial dilutions and the injected inoculum in triplicate.
Two volumes of BHI-NAD were added, and the cultures were incubated for an additional 100 min prior to plating out dilutions on selective and non-selective agar.
In order to test the ability of neurospheres to self-renew, primary neurospheres were dissociated to single cells and allowed to form secondary neurospheres prior to plating out in differentiation conditions.
Similar(32)
One day prior to transfection, ~12.105 cells were plated out on a single 15 cm petri dish.
This provides a measure of selectivity if there are two or more metals that can plate out; the metal ion that is more easily reduced can often be plated out quantitatively without any of the other metal also plating out.
Thus, samples were plated out on LB agar plates and incubated at 30°C for 6 d.
Five randomly selected, transformed colonies were able to grow when plated out again on hygromycinB-containing agar.
Hepatocytes were then plated out in supplemented media.
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