Furthermore, preliminary results from pulse plating experiments with ternary magnetic alloys, comprising 50 60% Co, 25 35% Fe and 10 20% Ni, will be reported.
Serial dilution and plating experiments showed that the erg26 mutation did indeed result in an elevated petite frequency at both 25° and 30° (Table 2).
The final percent growth inhibition at each concentration was averaged from six replicates of two independently plated experiments (n = 12).
The plating experiments were carried out in ambient condition at room temperature.
Plating experiments were performed on 300 mm diameter wafers with 4 nm, 5 nm and 10 nm thin Cu seed layers.
Dilution plating experiments were performed using 5 µl cells per spot, starting with an OD600 of 0.1 and serially diluting 5 times each step.
The increased fluorescence intensity can be attributed to increased eDNA levels in the matrix as dilution plating experiments revealed similar viable cell densities between these strains (data not shown).
For interconversion and clonal plating experiments, cells were treated for 30 minutes with the ROCK inhibitor Y-27632 (10 μM; Calbiochem) before dispersal and for 24 hours after sorting.
Replica plating experiments demonstrated that 9 of the 11 fidaxomicin-treated patients with new detection of isolates with fidaxomicin MICs >32 µg/mL had subpopulations (1%–10%) of VRE with similarly elevated MICs in samples prior to treatment.
For clonal plating experiments in vitro-derived NMPs were pre-treated with 10 µM ROCK inhibitor Y-27632 (Calbiochem) for 1 hr prior to fluorescence-activated cell sorting (FACS).
A pin-on-disk plate experiment is reconstructed to assess the efficiency and validity of the algorithms proposed here.
